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. 2023 Jan 23;9(2):e13190. doi: 10.1016/j.heliyon.2023.e13190

Table 1.

Primers for the CRISPR-based SARS-CoV-2 test.

Upstream primer (5′-3′) Downstream primer (5′-3′) CRISPR RNA (5′-3′) ssDNA
N N–F: ATGGAAGTCACACCTTCGGGAACGTGGTTG N-R: GGCTCTGTTGGTGGGAATGTTTTGTATGCG SARS-CoV2-N-CRISPR RNA: UAAUUUCUACUAAGUGUAGAUAAAGAUCAAGUCAUUUUGCUGAA TTATT
RNP Rnase P–F: GATCCGCAATAACTCAGCCATCCACATCCG Rnase P-R: CCCACCAAGAGACAATTACCCCCACCCTCA Rnase P- CRISPR RNA: AUUUCUACUAAGUGUAGAUAAUUACUUGGGUGUGACCCUGAA