KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| GFP | Chromotek | Cat# PABG1-100 |
| Tubulin | Cell Signaling Technologies | Cat# 3873 |
| Poly/Mono-ADP Ribose | Cell Signaling Technologies | Cat# 8372 |
| IRF3 | Cell Signaling Technologies | Cat# 11904 |
| p-IRF3 | Cell Signaling Technologies | Cat# 4947 |
| STAT1 | Cell Signaling Technologies | Cat# 9172 |
| p-STAT1 | Cell Signaling Technologies | Cat# 7649 |
| TBK-1 | Cell Signaling Technologies | Cat# 3504 |
| p-TBK-1 | Cell Signaling Technologies | Cat# 5483 |
| STING | Cell Signaling Technologies | Cat# 13647 |
| PARP7 | Rasmussen et al, 202138 | N/A |
| PARP1 | Cell Signaling Technologies | Cat# 9532 |
| GAPDH | Cell Signaling Technologies | Cat# 5174 |
| Actin | Santa Cruz Biotechnology | Cat# sc-47778 |
| His-Tag | Invitrogen | Cat# MA1135A647 |
| Rabbit-HRP | Jackson Immuno Research Labs | Cat# 111035144 |
| Mouse-HRP | Fisher Scientific | Cat# 62-6520 |
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 | Invitrogen | Cat# A-21235 |
| Bacterial and Virus Strains | ||
| Escherichia coli BL21 (DE3) competent cells | New England BioLabs | Cat# C2527H |
| ISRE Luciferase Reporter Lentivirus | BPS Bioscience | Cat# 79824 |
| HiBiT-Tag | Promega | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2’3’-cGAMP | Invivogen | tlrl-nacga23-02 |
| 3p-hp-RNA | Invivogen | tlrl-hprna |
| 6-alkyne-nicotinamide adenine dinucleotide (N-6 alkyne-NAD+) | Jiang et al, 201039; also commercially available from Biolog Life Science Institute | N/A; Biolog Cat# N051 |
| Ampicillin | Fisher Scientific | Cat# BP1760-5 |
| Bacto Tryptone | BD Biosciences | Cat# 211699 |
| Biotin-PEG3-azide | Click chemistry tools | Cat# AZ104 |
| Chloramphenicol | Fisher Scientific | Cat# BP904-100 |
| Deoxyribonucleic acid from calf thymus | Sigma-Aldrich | Cat# D4522 |
| Dextrose | Fisher Scientific | Cat# D16500 |
| DNAse I | Roche | Cat# 10104159001 |
| Dnick 5’P for PARP3 activation36 | IDT | Custom |
| Glycine | Fisher Scientific | Cat# BP381-1 |
| HEPES | Fisher Scientific | Cat# BP310-1 |
| Kanamycin | Fisher Scientific | Cat# BP9065 |
| NP-40 Substitute | Fisher Scientific | Cat# 507517565 |
| Phosphatase inhibitor cocktails 2 and 3 | Sigma | Cat# P5726-1ML, Cat# P0044-1ML |
| Potassium phosphate dibasic | Sigma-Aldrich | Cat# P3786-500g |
| Potassium phosphate monobasic | Sigma-Aldrich | Cat# P9791-100G |
| Sodium phosphate dibasic | Fisher Scientific | Cat# S472-500 |
| Strep-HRP | Fisher Scientific | Cat# NC9705430 |
| TCEP | Fisher Scientific | Cat# PI-20490 |
| Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA) | Click chemistry tools | Cat# 1061-100 |
| Triton X-100 | Fisher Scientific | Cat# BP151-500 |
| Yeast extract | Fisher Scientific | Cat# DF0127-07-1 |
| Yeast extract | Fisher Scientific | Cat# DF0127-07-1 |
| β-mercaptoethanol | Sigma-Aldrich | Cat# M3148-100mL |
| β-Nicotinamide adenine dinucleotide | Fisher Scientific | Cat# AAJ6163803 |
| 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) | Accustandard | Cat# D-404N |
| RBN-2397 | Custom Synthesis | N/A |
| DMSO | Fisher Scientific | Cat# BP231-100 |
| Paraformaldehyde | Fisher Scientific | Cat# 15713S |
| Sodium Azide | Fisher Scientific | Cat# S227I-25 |
| Horse Serum | Thermo Fisher | Cat# 16050122 |
| Citifluor CFMR2 Antifade | Fisher Scientific | Cat# 50-192-7400 |
| CoverGrip | Biotium | Cat# 23005 |
| KMR-206 | This Paper | N/A |
| Critical Commercial Assays | ||
| Aurum™ Total RNA isolation kit | BioRad | Cat# 7326820 |
| QuantaRed™ Enhanced Chemifluorescent HRP Substrate | Thermo-Fisher | Cat# 15159 |
| Mouse IFN Beta ELISA Kit | Fisher Scientific | Cat# 424001 |
| CalPhos Mammalian transfection kit | Fisher Scientific | Cat# NC9567834 |
| ONE-Glo Luciferase Assay System | Promega | Cat# E6110 |
| CellTiter-Glo™ 2.0 Assay | Promega | Cat# G9242 |
| jetOptimus DNA Transfection reagent | Polyplus | Cat# 117-07 |
| SuperSignal West Pico | Fisher Scientific | Cat# PI34080 |
| SuperSignal West Femto | Fisher Scientific | Cat# PI34095 |
| Experimental Models: Cell Lines | ||
| CT-26 | ATCC | Cat# CRL-2683 |
| MEFs | MacPherson et al, 20138 | N/A |
| HEK293T | ATCC | Cat# CRL-3216 |
| HeLa | ATCC | Cat# CCL-2 |
| NCI-H1373 | ATCC | Cat# CRL-5866 |
| Oligonucleotides | ||
| TBP FWD: 5’-TTGTACCGCAGCTGCAAAAT-3’ | MacPherson et al, 20138 | N/A |
| TBP REV: 5’-TATATTCGGCGTTTCGGGCA-3’ | MacPherson et al, 20138 | N/A |
| CYP1A1 FWD: 5’-TGGTCTCCCTTCTCTACACTCTTGT-3’ | MacPherson et al, 20138 | N/A |
| CYP1A1 REV: 5’-ATTTTCCCTATTACATTAAATCAATGGTTCT-3’ | MacPherson et al, 20138 | N/A |
| IFN-B FWD: 5’-TGGGAGATGTCCTCAACTGC-3’ | MacPherson et al, 20138 | N/A |
| IFN-B REV: 5’-CCAGGAGTAGCTGTTGTACT-3’ | MacPherson et al, 20138 | N/A |
| Recombinant DNA | ||
| GFP-PARP7 | Rodriguez et al, 202113 | N/A |
| mRuby2-Nup50-N-10 | Addgene | Plasmid# 55908 |
| Software and Algorithms | ||
| GraphPad Prism 9 | GraphPad | N/A |
| Other | ||
| Ni-NTA Agarose | Qiagen | Cat# 30210 |
| 96-well Ni-NTA coated plate | Thermo Scientific | Cat# 15242 |
| 96-well Glutathione coated plate | Thermo Scientific | Cat# 15240 |