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. Author manuscript; available in PMC: 2024 Jan 19.
Published in final edited form as: Cell Chem Biol. 2023 Jan 13;30(1):85–96.e6. doi: 10.1016/j.chembiol.2022.12.005

Figure 4. Evaluation of GPRC5D-RAMIHM humanized mice and rapidly identified fully human GPRC5D-specific monoclonal antibodies.

Figure 4.

A, The structure of human GPRC5D protein predicted (UniProt #Q9NZD1) by AlphaFold Protein Structure Database.

B, GPRC5D antibody titer measurement in plasma samples. All plasma samples were diluted into 1:200, then incubated with K562-GPRC5D O/E cells and detected by flow cytometry. Data from two CD22-RAMIHM mice were shown.

C, Single B cell variable chains for antibody cloning. Variable (V) genes and CDR3 lengths for paired heavy- and light-chains of top-enriched clones to GPRC5D from single BCR sequencing.

D, Distribution of heavy chain complementarity-determining region 3 (HCDR3) length in memory B cells from GPRC5D-RAMIHM mouse.

F, Global frequencies of IGHV, IGHJ, IGKV, and IGKJ genes usage in GPRC5D-RAMIHM mouse.

G, Numbers, and diversifications of IGHV/IGHJ, IGKV/IGKJ gene combination usage in GPRC5D-RAMIHM mouse.

See also Figures S5–S7