Figure 4. The majority of genome-edited cells following NC delivery are neuronal.

Genome-edited, tdTomato-expressing (red) cells in the striatum of representative animals from each of the NC treatment groups (NC-No Ligand animal J12; NC-CPP animal J18; NC-RVG animal J5; Supp. Table 1). Scale bar = 100 μm. Arrowheads point to examples of tdTomato+/NeuN+ genome-edited neurons (yellow in 2^nd column). Arrows point to tdTomato+/GFAP+ genome-edited astrocytes (yellow in 4^th column). Photomicrographs show maximum intensity projection of three focal planes covering 10 μm. Individual channels were adjusted for brightness as needed (Supp. Table 4). CPP, cell-penetrating peptide; DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein; NC, nanocapsule; NeuN, neuronal nuclear protein; RVG, rabies virus glycoprotein.