FIG. 2.

Neutralization of TNF-α or IL-18 inhibits M. bovis BCG-induced MMP secretion by macrophages. Macrophage monolayers were incubated with either medium alone (MØ) or with 0.1 BCG bacterium per macrophage (MØ BCG) in the absence or presence of neutralizing antibodies to TNF-α, IL-18, IL-12, GM-CSF, or the IL-1β receptor CD121a (MØ BCG Ab), or relevant isotype control antibodies (MØ BCG control). Cell culture supernatants were harvested after 72 h and analyzed for gelatinase activity using zymography. Data presented are representative of two to four independent experiments.