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. 2023 Jan 9;13:1038802. doi: 10.3389/fphar.2022.1038802

FIGURE 6.

FIGURE 6

AMPK phosphorylation accelerates UCP2 protein expression in SAE models. (A) Phospho-AMPKα protein expression levels in the cerebrum tissues were detected using immunoblotting assays. GAPDH was used as an internal reference. (B) Gray ratio analysis of UCP2/GAPDH in (A) using ImageJ and excel software (n = 3). (C) Phospho-AMPKα and UCP2 protein expression levels in BV-2 cells pretreated with or without AMPK inhibitor dorsomorphin. (D) Gray ratio analysis of Phospho-AMPKα/GAPDH in (C) (n = 3). (E) Gray ratio analysis of UCP2/GAPDH in (C) (n = 3). Statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc testing. * represents comparison between LPS group and control group, # represents comparison among malvidin groups and LPS group in (B) or between dorsomorphin group and control group in (D,E), whereas & indicates analysis among LPS injection combined with malvidin pretreatment groups with or without dorsomorphin pretreatment groups in (D, ). */#/& is p < 0.05, && is p < 0.01, ***/&&& is p < 0.001, and ns is p > 0.05.