Skip to main content
. 2023 Jan 9;13:1038802. doi: 10.3389/fphar.2022.1038802

FIGURE 7.

FIGURE 7

Malvidin protects against LPS induced SAE mice cerebrum inflammation through inhibiting ROS-mediated NLRP3 inflammasome activation. (A) Pro-inflammatory cytokines IL-6 (A), TNF-α (B), IL-1β (C) secretion and LDH (D) release levels in the SAE mice serum. (E) The expression levels of NLRP3 inflammasome releated proteins of NLRP3, ASC and caspase-1 in the cerebrum tissues using immunoblotting assays. GAPDH was used as an internal reference. Gray ratio analysis of NLRP3/GAPDH (F), ASC/GAPDH (G) and caspase-1/GAPDH (H) in (E) using ImageJ and excel software (n = 3). (I) The expression levels of NLRP3 inflammasome releated proteins of NLRP3, ASC and caspase-1 in BV-2 cells lysates using immunoblotting assays. GAPDH was used as an internal reference. Gray ratio analysis of NLRP3/GAPDH (J), ASC/GAPDH (K), and caspase-1/GAPDH (L) in (I) using ImageJ and excel software (n = 3). Statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc testing. * represents comparison between LPS group and control group, # represents comparison among malvidin groups and LPS group in (A–D, F–H) or between NAC group and control group in (J–L), whereas & indicates analysis among LPS injection combined with malvidin pretreatment groups with or without NAC pretreatment groups in (J–L). */# is p < 0.05, **/##/&& is p < 0.01, ***/###/&&& is p < 0.001, and ns is p > 0.05.