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. 2023 Jan 9;13:1038802. doi: 10.3389/fphar.2022.1038802

FIGURE 8.

FIGURE 8

Malvidin protects against LPS induced mice cerebrum injury through inhibiting ROS-mediated apoptosis. (A) TEM observation of the microstructures of the nucleus of the cerebral cortex. (B) Tunel staining of apoptosis cells in the cerebrum. Scale bar = 100 µm. The tunnel positive cells were stained in red and nuclear is stained with DAPI (in blue). (C) The expression levels of apoptosis related proteins of Bax, Bcl-2, Cyto-C and caspase-3 in the cerebrum tissues using immunoblotting assays. GAPDH was used as an internal reference. Gray ratio analysis of Bax/GAPDH (D), Bcl-2/GAPDH (E), Cyto-C/GAPDH (F), caspase-3/GAPDH (G) in (C) using ImageJ and excel software (n = 3). (H) The expression levels of apoptosis related proteins of Bax and caspase-3 in the BV-2 cell lysates using immunoblotting assays. GAPDH was used as an internal reference. Gray ratio analysis of Bax/GAPDH (I), and caspase-3/GAPDH (J) in (H) using ImageJ and excel software (n = 3). * represents comparison between LPS group and control group, # represents comparison among LPS injection group and malvidin groups or between NAC group and control group, & indicates comparison among malvidin treatment followed by LPS injection groups with or without NAC pretreatment groups. */#/& is p < 0.05, **/## is p < 0.01, ***/###/&&& is p < 0.001, and ns is p > 0.05.