FIG. 4.

Capture ELISA of AM in M. tuberculosis culture medium. M. tuberculosis Erdman and CDC 1551 were grown in the absence or presence of Tween 80 for periods of 5, 11, 15, 20, or 25 days before harvest of bacterial supernatant. Each value represents the average of two separate measurements. Error bars represent the standard deviation of the means. These growth experiments and the ELISA were repeated twice with similar results. The detection limit of the assay is indicated by the dotted line. Alk-Phos, alkaline phosphatase.