Table 2.
Nucleotide sequences of primers for quantitative real-time PCR assay.
| Name | Primer sequence | GenBank accession |
|---|---|---|
| Receptors | ||
| TLR-4 | F: CCACTATTCGGTTGGTGGAC | NM_001030693.1 |
| R: ACAGCTTCTCAGCAGGCAAT | ||
| Adaptor proteins | ||
| MyD88 | F: GGATGGTGGTCGTCATTTCA | NM_001030962.1 |
| R: GAGATTTTGCCAGTCTTGTCCA | ||
| NF-κB | F: TGGAGAAGGCTATGCAGCTT | NM_205134.1 |
| R: CATCCTGGACAGCAGTGAGA | ||
| Pro-inflammatory cytokines | ||
| TNF-α | F: CCCCTACCCTGTCCCACAA | NM204267 |
| R: TGAGTACTGCGGAGGGTTCAT | ||
| IL-1β | F: CAGCAGCCTCAGCGAAGAG | NM_204524.1 |
| R: CTGTGGTGTGCTCAGAATCCA | ||
| IL-8 | F: GGCTTGCTAGGGGAAATGA | AJ009800 |
| R: AGCTGACTCTGACTAGGAAACTGT | ||
| IFN-γ | F: AAAGCCGCACATCAAACACA | NM_205149.1 |
| R: GCCATCAGGAAGGTTGTTTTTC | ||
| Ant-inflammatory cytokines | ||
| IL-10 | F: CGCTGTCACCGCTTCTTCA | NM_001004414.2 |
| R: CGTCTCCTTGATCTGCTTGATG | ||
| Tight junctions | ||
| Claudin-1 | F: AAGTGCATGGAGGATGACCA | NM_001013611.2 |
| R: GCCACTCTGTTGCCATACCA | ||
| Occlaudin | F: TCATCGCCTCCATCGTCTAC | NM_205128.1 |
| R: TCTTACTGCGCGTCTTCTGG | ||
| ZO-1 | F: TATGAAGATCGTGCGCCTCC | XM_015278981.1 |
| R: GAGGTCTGCCATCGTAGCTC | ||
| Mucin-2 | F: AGCGAGATGTTGGCGATGAT | NM_001318434.1 |
| R: AAGTTGCCACACAGACCACA | ||
| Growth factors | ||
| TGF-β3 | F: TGCGGCCAGATGAGCAT | NM_205454.1 |
| R: TGCACATTCCTGCCACTGA | ||
| IGF-2 | F: TGGCTCTGCTGGAAACCTAC | NM_001030342.2 |
| R: ACTTGGCATGAGATGGCTTC | ||
| EGFR | F: ACCAGCCTGCAGAGAATGTA | NM_205497 |
| R: CACCATGTTAAGCGCAATGA | ||
| GLP-2 | F: AAGCTTCCCAGTCTGAACCA | NM_205260.4 |
| R: ATCCTGAGCTCGTCTGCTGT | ||
| House-keeping genes | ||
| β-actin | F: GAGAAATTGTGCGTGACATCA | NM 205518 |
| R: CCTGAACCTCTCATTGCCA |
TLR = toll-like receptor; MyD88 = myeloid differential protein-88; NF-κB = nuclear factor kappa-light-chain-enhancer of activated B cells; TNF-α = tumor necrosis factor α; IL = interleukin; IFN-γ = interferon γ; ZO-1 = zonula occludens-1; EGFR = epidermal growth factor receptor; GLP-2 = glucagon like peptide-2; IGF-2 = insulin-like growth factor-2; TGF-β3 = transforming growth factor beta 3.