Table 7.
Effects of dietary coated essential oil and organic mixture supplementation on gene expressions of TLR signaling pathway-related genes in the jejunum of broiler chickens challenged with E. coli O78.
| Item | E. coli1 | TLR-4 | MyD88 | NF-κB | IFN-γ | TNF-α | IL-1β | IL-8 | IL-10 |
|---|---|---|---|---|---|---|---|---|---|
| EOA, mg/kg | |||||||||
| 0 | - | 1.07 | 1.02 | 1.06 | 1.01 | 1.00 | 1.05 | 1.04 | 1.07b |
| 0 | + | 1.92 | 1.18 | 1.04 | 2.74 | 1.04 | 1.12 | 1.38 | 0.72b |
| 500 | - | 1.18 | 1.11 | 1.16 | 1.13 | 1.32 | 1.02 | 0.75 | 0.81b |
| 500 | + | 1.31 | 1.22 | 1.03 | 1.06 | 1.13 | 1.09 | 1.96 | 1.84a |
| SEM2 | 0.10 | 0.08 | 0.09 | 0.09 | 0.06 | 0.14 | 0.32 | 0.11 | |
| Main effect | |||||||||
| 0 | 1.50 | 1.10 | 1.05 | 1.87 | 1.02 | 1.09 | 1.21 | 0.90 | |
| 500 | 1.20 | 1.17 | 1.10 | 1.09 | 1.23 | 1.05 | 1.36 | 1.33 | |
| Non-challenged | 1.13 | 1.07 | 1.11 | 1.07 | 1.16 | 1.04 | 0.90 | 0.94 | |
| Challenged | 1.66 | 1.20 | 1.03 | 1.90 | 1.09 | 1.10 | 1.67 | 1.28 | |
| P-value2 | |||||||||
| EOA | 0.377 | 0.663 | 0.805 | 0.408 | 0.095 | 0.905 | 0.821 | 0.055 | |
| Challenged | 0.032 | 0.407 | 0.676 | 0.016 | 0.524 | 0.803 | 0.013 | 0.126 | |
| Challenged × EOA | 0.567 | 0.877 | 0.747 | <0.01 | 0.353 | 0.998 | 0.237 | <0.01 |
TLR = toll-like receptor; MyD88 = myeloid differential protein-88; NF-κB = nuclear factor kappa-light-chain-enhancer of activated B cells; TNF-α = tumor necrosis factor α; IL = interleukin; IFN-γ = interferon γ; SEM = standard error of the mean.
a, b Means within the same column with different superscripts differ significantly (P < 0.05).
“–”, without E. coli O78 challenge; “+”, with E. coli O78 challenge.
P-values represent the main effect of the diet, the main effect of E. coli O78 challenge, and the interaction between the dietary treatments and E. coli O78 challenge.