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. 2023 Jan 9;10:1091629. doi: 10.3389/fcell.2022.1091629

FIGURE 5.

FIGURE 5

Apical constriction within the neuroepithelium is defective when somitic mesoderm morphogenesis is impaired. (A) Maximum intensity projection image of a representative stage 16 embryo. Asterisk: Wee2 somitic mesoderm morphant side. Scale bar: 100 μm (A’) Zoomed images of control neuroepithelial cells and neuroepithelial cells overlying Wee2 morphant somitic mesoderm. The apical surface area of neural plate cells overlying the morphant somitic mesoderm side is larger compared to that of control cells. (B) Quantification of apical cell surface area. Two-sided unpaired Student’s t-test; ****p < .0001; mean ± SEM; n = 150 cells. (C) Quantification of apical cell surface area over time from neuroepithelial cells in Movie 4 and panel (D); Two-sided unpaired Student’s t-test; ****p < .0001; n = 20 cells for each time point. Neuroepithelial cells overlying Wee2 morphant somitic mesoderm fail to undergo apical constriction (D) Stills from a time lapse recording of a neurula embryo with unilateral Wee2 morphant somitic mesoderm. Red dotted line: neural plate midline. Asterisk: Wee morphant side. Scale bar: 50 μm.