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. 2022 Dec 28;25(1):1–10. doi: 10.22074/cellj.2022.8315

Fig 3.

Fig 3

The effects of various doses of VCR and ASP on viability of ALL cell lines. A. MTT test was performed to investigate the survival of ALL cell lines at 48 hours after treatment with VCR and ASP. B. Staining with Annexin-V and PI assessed apoptosis. The histogram demonstrates the ratio of apoptotic or necrotic cells. Annexin V-/PI-, Annexin V+/PI-, Annexin V+, and PI+ represented live cells, early apoptotic cells, late apoptotic cells, necrotic cells, respectively. The data are from two independent experiments, each done in duplicate. VCR; Vincristine, ASP; L-asparaginase, ALL; Acute lymphoblastic leukemia, MTT; Dimethylthiazol diphenyl tetrazolium bromide, VCR; Vincristine, ASP; L-asparaginase, and PI; Propidium iodide.