TABLE 1.
E. tarda strains and their percentage of adherence to phagocytes
E. tarda strain | Inoculum (105) | Adherence at
25°Ca
|
|||
---|---|---|---|---|---|
Viable count
(%)b
|
Direct microscopic count
(%)c
|
||||
Nonopsonized | Opsonized | Nonopsonized | Opsonized | ||
PPD130/91 | 7.3 ± 1.3 | 8.8 ± 0.5 Aα | 11.7 ± 0.8 Bα | 10.9 ± 1.4 Aγ | 14.4 ± 1.6 Aγ |
AL9379 | 8.2 ± 1.3 | 22.9 ± 0.6 Cα | 38.2 ± 5.2 Dβ | 47.8 ± 2.0 Cγ | 68.1 ± 3.1 BCδ |
PPD76/87 | 6.4 ± 1.0 | 12.5 ± 0.6 Bα | 0.02 ± 0.01 Aβ | 37.7 ± 2.6 Bγ | 78.9 ± 6.1 Cδ |
PPD125/87 | 9.8 ± 1.5 | 33.0 ± 2.3 Dα | 21.0 ± 1.6 Cβ | 57.1 ± 1.9 Dγ | 65.1 ± 0.7 Bδ |
Within each column, values followed by different letters (A, B, C, or D) are significantly different (P < 0.05). Similarly, within each row, values followed by different Greek letters (α and β for viable counts and γ and δ for direct counts) are significantly different (P < 0.05).
The viable count is the percentage of inoculum bacteria still adherent 30 min postinfection and after being washed three times. Values were recorded as the mean ± SEM for three trials in duplicate wells.
The direct microscopic count is the number of adherent bacteria (both live and dead) per 100 phagocytes 30 min postinfection. Values were recorded as the mean ± SEM for three trials in duplicate coverslips.