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. 2023 Jan 5;26(2):105932. doi: 10.1016/j.isci.2023.105932

Figure 5.

Figure 5

NEAT1 elevated PDPK1 expression through targeting miR-147 after radiation in BNL CL2 cells

(A) The expression of PDPK1 mRNA at the different time points after radiation.

(B) The expression of PDPK1 protein at the different time points after radiation.

(C) The expression of AKT protein at the different time points after radiation.

(D) The relative expression of PDPK1 mRNA significantly increased in the NEAT1 group.

(E) The relative expression of PDPK1 protein significantly increased in the NEAT1 group.

(F) The relative expression of AKT1 mRNA significantly increased in the NEAT1 group.

(G) The relative expression of AKT1 protein significantly increased in the NEAT1 group.

(H) The relative expression of PDPK1 mRNA decreased in the miR-147 group, compared with NC, and increased in the miR-147 + NEAT1 group, compared with miR-147 + NC.

(I) The inhibition of miR-147 on PDPK1 was significantly neutralized by NEAT1. In the group of NEAT1 MUT, the effect was canceled.

(J) Effect of TRT on the expression of PDPK1 mRNA after transfection of NEAT1 and radiation injury.

(K) Effect of TRT on the expression of PDPK1 protein after transfection of NEAT1 and radiation.

(L) Impact of TRT on the expression of PDPK1 mRNA after transfection of NEAT1 shRNA and radiation injury.

(M) Effect of TRT on the expression of PDPK1 protein after the transfection of NEAT1 shRNA and radiation injury. n = 3. ∗p < 0.01; NS: not significant. Data were shown as mean ± SEM.