REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-AKT	Proteintech Group Inc.	Cat#10176-2-AP; RRID: AB_2224574
Anti-p-AKT	Proteintech Group Inc.	Cat#66444-1-1g; RRID: AB_2919327
Anti-p-JNK	Wan-Lei Biotechnology Co., Ltd.	Cat#WL01813; RRID: AB_2910628
Anti-α-Actintin	Proteintech Group Inc.	Cat#11313-2-AP; RRID: AB_2223815
Anti-GAPDH	Proteintech Group Inc.	Cat#10494-1-AP; RRID: AB_2263076
Anti-PDPK1	Proteintech Group Inc.	Cat#17086-1-AP; RRID: AB_2161289
Goat anti-rabbit IgG-Cy3	Bi-Yun-Tian Biotechnology Co., Ltd.	Cat#A0516; RRID: AB_2893015
Horseradish peroxidase (HRP)-labeled antibody	Cell Signaling Technology	Cat#7074S; RRID: AB_2099233
Chemicals, peptides, and recombinant proteins
Troxerutin	MedChemExpress	Lot#27664
TRIzol	Life Technologies	Lot#350302
Critical commercial assays
ReverTra Ace qPCR RT Master Mix kit	Toyobo, Tonga,Japan	FSQ-201
RT First Strand cDNA Synthesis Kit	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	G3330-50
SYBR1 Green Real-time PCR Master Mix kit	Toyobo, Tonga,Japan	QPK-201
A protease inhibitor cocktail was obtained from Roche Diagnostics GmbH	Mannheim, Germany	11836145001
TurboFect Transfection Reagente	Thermo Fisher Scientific, America	R0531
PMSF	Bi-Yun-Tian Biotechnology Co., Ltd.	ST506
BCA protein concentration determination kit BCA protein concentration determination kit	Bi-Yun-Tian Biotechnology Co., Ltd.	P0010
Beyo ECL Plus	Bi-Yun-Tian Biotechnology Co., Ltd.	P0018m
Experimental models: Cell lines
BNL CL2	Beina Chuanglian Biotechnology Co., Ltd.	No.: BNCC 337873
L02	Beina Chuanglian Biotechnology Co., Ltd.	No.: BNCC 351907
MTEC-1	Shanghai Hongshun Biotechnology Co., Ltd.	No.: HSC9942
V79	Beijing Dingguo Changsheng Biotechnology Co., Ltd.	No.: CS0199
Experimental models: Organisms/strains
C57BL/6N mouse	Beijing Wei-tong-li-Hua Experimental Animal Technology Co.	http://www.lascn.com/Category_1785/Index.aspx
Pre-mir-147−/− mouse	Chinese-French Immunoregulatory Genes Laboratory of Xinxiang Medical University	MGI number: 2676832
Oligonucleotides
H-NEAT1- sequencing: Forward:5′-GGGGTGGTCTGAGGAGTGATG-3′ Reverse:5′-CATTACCAACAATACCGACTCCAA-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
M-Neat1 sequencing: Forward:5′-TAGGTTCCGTGCTTCCTCTTCT-3′ Reverse:5′-CCCTCTGTGAATCAACACCATC-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
mmu-miR-147-3p-RT sequencing:5′-CTCAACTGGTGTCGTGGAGTCGGCA ATTCAGTTGAGTAGCAGAAGCATTT-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
mmu-miR-147-3p-Ssequencing:5′-GTGT GCGGAAATGCTTCTGCTA-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
hsa-miR-147a-RT sequencing:5′-CTCAACTGGTGTCGTGGAGTCGGCA ATTCAGTTGAGGCAGAAGC-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
hsa-miR-147a-S sequencing:5′-ACACTCCAG CTGGGGTGTGTGGAAATGC-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
universal primer-A sequencing:5′-TGGTGTCGTGGAGTCG-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
M-PDPK1 sequencing: Forward:5′-AGGAGGACGCTGAGGAGG-3′ Reverse:5′-AATGGGCACAGCGTCATACA-3′	Wuhan Servicebio Technology Co., Ltd.’ Wuhan, China	N/A
H-PDPK1 sequencing: Forward:5′-CAGAGGTCAGGCAGCAACATA-3′ Reverse:5′-CTGTTAGGCAAGGGTTTCCG-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
H-GAPDH sequencing: Forward:5′-GGAAGCTTGTCATCAATGGAAATC-3′ Reverse:5′-TGATGACCCTTTTGGCTCCC-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
M-GAPDH sequencing: Forward:5′-CCTCGTCCCGTAGACAAAATG-3′ Reverse:5′-TGAGGTCAATGAAGGGGTCGT-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
U6 sequencing: Forward:5′-CTCGCTTCGGCAGCACA-3′ Reverse:5′-AACGCTTCACGAATTTGCGT-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
SgRNA-1(matching forward strand of the gene)sequencing:5′-AGAACACTCTATGAATCTAG TGG-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
SgRNA-2(matching reverse strand of the gene)sequencing:5′-GCAGAAGCATTTCCGCACAC TGG-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
SgRNA-3(matching forward strand of the gene)sequencing:5′-GATCCTGCATTAGCAAGTGA AGG-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
(miR-147, genotyping) Forward primer sequencing:5′-TGAGGAGCTGCAAAAAGTCC-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
(miR-147, genotyping) Reverse primer sequencing:5′-CAAAATGTGCTTGTTCCCATAA-3′	Wuhan Servicebio Technology Co., Ltd. Wuhan, China	N/A
Recombinant DNA
mmu-miR-147-3p-inh-IMAT0004857(GV249)	Shanghai Genechem Co., Ltd.	GMDE0168564
mmu-mir-147-MI0005482(GV268)	Shanghai Genechem Co., Ltd.	GMUE0284900
hsa-miR-147a-inh-MIMAT0000251(GV249)	Shanghai Genechem Co., Ltd.	GMDE0189784
has-mir-147a-MI0000262(GV268)	Shanghai Genechem Co., Ltd.	GMUE0103344
AKT1-NM_009652(GV146)	Shanghai Genechem Co., Ltd.	GOSE0217154
AKT1 sh-NM_009652(GV102)	Shanghai Genechem Co., Ltd.	GIEE0217153
Pdpk1 sh-NM_011062(GV102)	Shanghai Genechem Co., Ltd.	GIEE0197550
Pdpk1-NM_011062(GV146)	Shanghai Genechem Co., Ltd.	GOSE0168969
NEAT1-NR_131012(miR-147a) (GV272)	Shanghai Genechem Co., Ltd.	GOSE0284892
NEAT1-NR_131012(miR-147a)-mut(GV272)	Shanghai Genechem Co., Ltd.	GOSE0284897
Neat1-NR_131012(miR-147-3p) (GV272)	Shanghai Genechem Co., Ltd.	GOSE0284902
Neat1-NR_131212(miR147-3p)mut (GV272)	Shanghai Genechem Co., Ltd.	GOSE0284903