FIGURE 4.

Immobilization of E. coli WT (−), E. coli (FL-ataA), and E. coli (mini-ataA) on the polyurethane foam support. (A) Photographs of the bacterial cell suspension after shaking for 60 min with the support. As a comparison, Tol 5, which originally expresses AtaA, is also shown. (B) Micrographs of polyurethane fibers after shaking with bacterial cells. The cells were stained with propidium iodide (red). Scale bars: 250 μm. (C) Immobilization ratio calculated from a decrease in the OD₆₀₀ of the cell suspension after shaking. The data are represented as the mean ± SEM (n = 3).