FIGURE 6.

Enzymatic reaction by E. coli JCM (bgl, mini-ataA) cells expressing mini-AtaA and β-glucosidase. (A) Growth curves of E. coli JCM (bgl, mini-ataA) and the control strain harboring an empty vector (bgl) in LB medium supplemented with arabinose for induction of the mini-ataA gene. (B) BGL activity of suspended cells of E. coli JCM (bgl, mini-ataA) and of the control strain (bgl) harboring an empty vector. Blank, without cells. (C) Immobilization ratio calculated from a decrease in the OD₆₀₀ of the cell suspension after shaking. (D,E) Repetitive BGL reaction using E. coli JCM (bgl, mini-ataA) cells. The support with the immobilized cells was placed in a test tube containing the reaction mixture. After every 50-min reaction, the support was collected, washed with BS-N buffer for 10 min, and transferred to a fresh reaction solution for the next new reaction. This process was repeated 5 times. Pictures of the test tubes before and after the 50-min reaction are shown in panel (D). The data are represented as the mean ± SEM (n = 3).