TABLE 2.
Oxidative stress markers and mitochondrial NO synthase (mtNOS) activity in heart mitochondria of adult, old and GSH-treated old rats.
| Adult (n = 9) | Old (n = 9) | Old + GSH (n = 9) | |
|---|---|---|---|
| O2•−, (nmol/min/mg protein) | 4.62 ± 0.22 | 16.87 ± 4.87* | 6.70 ± 0.38*,# |
| H2O2, (pmol/mg protein) | 12.36 ± 3.11 | 25.59 ± 3.24* | 11.06 ± 0.10# |
| MDA, (nmol/mg protein) | 2.11 ± 0.15 | 5.93 ± 0.36* | 3.71 ± 0.20# |
| DC, (ng/mg protein) | 3.73 ± 0.24 | 16.01 ± 2.25* | 7.70 ± 0.92# |
| mtNOS activity, (pmol/min/mg protein) | 4.5 ± 0.24 | 2.28 ± 0.39* | 8.62 ± 2.05# |
Note: GSH, glutathione; O2•−, Superoxide; H2O2, hydroperoxide; MDA, malondialdehyde; DC, diene conjugates.
*р < 0.05 versus adult.
# p < 0.05 versus old as calculated by ANOVA, test.