Figure 4.

Analysis of the cognitive behavior of 3 x Tg and APP/PS1 AD mice treated with 4c. (A) Schematic diagram of the experimental design for treating 3 x Tg-AD mice with 4c. Six groups of mice were treated every 2 days for 4 weeks: WT with 200 μg/kg 4c (WT-4c-H), WT with vehicle (WT), 3 x Tg with vehicle (3 x Tg), 3 x Tg with 20 μg/kg 4c (4c-L), 3 x Tg with 200 μg/kg 4c (4c-H), and 3 x Tg with 200 μg/kg Evo (Evo). (B) After treatment, all mice were subjected to the Y maze test and the percentage of alternations was calculated. (C) After the Y maze test, the mice were subjected to the MWM test and the latency to find the hidden platform during the 5-day training period was comparatively analyzed. (D, E) The number of platform crossings and time in the target quadrant during the probe trial on day 6 were recorded. (F–H) The distance, velocity, and track examples of the mice in the MWM test were recorded during the probe trial on day 6. Data are represented as mean ± SEM, n = 12; ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. (I) Schematic diagram of the experimental design for treating APP/PS1 AD mice with 4c. Five groups of mice were treated every 2 days for 1 month: WT with 200 μg/kg 4c (WT-4c-H), WT with vehicle (WT), APP/PS1 with vehicle (APP/PS1), APP/PS1 with 200 μg/kg 4c (4c-H), and APP/PS1 with 200 μg/kg Evo (Evo). (J) After treatment, working memory was assessed by performing the Y maze test and analyzing the percentage of alternations. (K–M) The treated mice were subjected to the MWM test and the latency to find the hidden platform during the 5-day training period was recorded. On day 6, the spatial memory of the mice was evaluated by monitoring the number of platform crossings and time in the target quadrant. (N–P) The distance, velocity, and track examples of the mice in the MWM were recorded on day 6. Data are represented as mean ± SEM, n = 10; ^*P < 0.05 and ^**P < 0.01.