Figure 6.

Observation of Aβ pathology in the brain in APP/PS1 mice. After the cognitive behavior evaluations, mice from the APP/PS1, 4c-H, and Evo groups were selectively sacrificed, and the paraffin sections of the brain were prepared by a standard pathological procedure. (A, B) Aβ in the cortex and hippocampus was observed by immunofluorescence staining with an anti-6E10 antibody (green) and counterstained with DAPI (scale bar = 200 μm). (C–F) The Aβ plaque area and plaque counts in the cortical and hippocampal regions were quantified by ImageJ (n = 9). (G, H, K, L) Microglia around Aβ plaques were observed by double immunofluorescence staining with anti-6E10 (green) and anti-Iba1 (red) antibodies in the cortex and hippocampus. The cell number of microglia around Aβ plaques was also counted (scale bar = 10 μm, n = 6). (I, J, M, N) The astrocytes around Aβ plaques were observed by double immunofluorescence staining with anti-6E10 (green) and anti-GFAP (red) antibodies in the cortex and the hippocampus, and the number of astrocytes around Aβ plaques was counted (scale bar = 10 μm, n = 6). (O, P) Low-molecular weight oligomers (LMW; 1–5 mers) of Aβ in brain tissues were detected by western blotting and quantified using ImageJ (n = 3). Data are represented as mean ± SEM; ^*P < 0.05 and ^***P < 0.001.