Table 1.
Antibodies used in this study.
| Primary antibodies | Source | Catalog no. | WB | IHC | IF |
|---|---|---|---|---|---|
| 6E10 | Biolegend | 803001 | 1:500 | — | 1:150 |
| IBA1 | CST | 17198 | — | — | 1:150 |
| GFAP | Abcam | ab7260 | — | — | 1:200 |
| T231 (p-TAU at Thr231) | Abcam | ab151559 | — | 1:200 | — |
| T181 (p-TAU at Thr181) | Abcam | ab75679 | 1:500 | 1:200 | 1:100 |
| AT8 (p-TAU at Ser202/Thr205) | Thermo Fisher | MN1020 | 1:500 | — | 1:100 |
| p-PI3K | CST | 4228 | 1:500 | — | — |
| PI3K | CST | 4292S | 1:500 | — | — |
| p-AKT (Ser473) | CST | 9271 | 1:1,000 | — | — |
| AKT | CST | 4691s | 1:1000 | — | — |
| p-GSK3α/β (Ser21/9) | CST | 9331s | 1:1,000 | — | — |
| GSK3β | CST | 9315 | 1:1,000 | — | — |
| GAPDH | Abcam | ab201822 | 1:10,000 | — | — |
| TAU5 | Millipore | 577801 | 1:500 | — | — |
| 488 goat anti-mouse IgG | Invitrogen | A11029 | — | — | 1:200 |
| 555 goat anti-rabbit IgG | Invitrogen | A21429 | — | — | 1:200 |
| HRP-anti-mouse IgG | ZSGB-BIO | PV9002 | — | 1:20 | — |
| HRP-t anti-rabbit-IgG | ZSGB-BIO | PV9001 | — | 1:20 | — |