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. 2022 Nov 19;9(4):610–616. doi: 10.5455/javar.2022.i630

Table 3. Data on HPLC with retention time and wavelength values of standard compounds and compounds in seahorse extracts.

Standard compounds/extract Retention time UV absorbance (nm) mAU
Ethinyl estradiol 4.100 248.79; 279.98; 356.13 300
Methyl testosterone 3.500 208.13; 240.93; 282.68; 374.48; 486.99; 521.75; 654.97; 749.18 300
Progesterone 7.408 202.78; 242.29; 361.05 50
Ethanol extract of CS 3.005
2.705
2.555
239.4; 347.76
485.83; 550.37; 577.35; 666.93; 738.28.
-
110
300
200
Water extract of CS 2.549
2.693
2.566
248.84; 275.66
249.13; 273.64; 485.76; 514.11; 581.14; 631.80; 665.5; 756.46
248.61; 274.86
350
600
350
Ethanol extract of NS 2.510
2.672
3.057
240.74; 251.75; 350.73
193.36; 240.2; 424.99; 485.39; 581.03; 663.85
239.65; 250.69; 355.01
70
150
50
Water extract of NS 2.472
2.707
2.580
252.81; 274.91
195.51; 252.39; 467.55; 581.54; 631.77; 655.6; 748.03
253.64; 274.48
750
1,000
500