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. 2022 Dec 22;22(2):100489. doi: 10.1016/j.mcpro.2022.100489

Fig. 3.

Fig. 3

Parametrization of synchro-PASEF scan modes.A, acquisition schemes for synchro scan methods with different quadrupole selection window widths. B, cycle times of the methods in (A). C, precursor coverage of methods in (A) calculated with the HeLa reference library. D, quadrupole step size in dependence on the TIMS ramp time. E, precursor elution profiles and sampled data points in the retention time dimension at a throughput of 60 SPD (21 min), 100 (11 min), 200 (5.6 min), and 300 (3.2 min) for the peptide GLAGVENVTELK. PASEF, parallel accumulation—serial fragmentation; SPD, samples per day; TIMS, trapped ion mobility spectrometry.