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. 2023 Jan 12;49(2):43. doi: 10.3892/or.2023.8480

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Copyright: © Lim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of ACT-3 on HDAC expression levels in MCF-7 cells. (A) Effects of ACT-3, suramin, and SAHA on total HDAC enzyme activity. HDAC enzyme activity was measured using the SensoLyte 520 FRET total HDAC assay kit. (B and C) MCF-7 cells were treated with ACT-3 and SAHA for 48 h and expression levels of Ac-H3, HDAC1, HDAC3 and Histone H1 were analyzed via western blotting. The data are expressed as the mean ± SD of duplicate experiments. Statistical analysis was performed using one-way analysis of the variance (ANOVA), followed by Bonferroni's multiple comparison tests. *P<0.05 and **P<0.01 vs. the control. ACT-3, 3β,6β-dihydroxyurs-12-en-27-oic acid; HDAC, histone deacetylase; SAHA, suberoylanilide hydroxamic acid; Ac-H3, acetylated histone 3.