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. 2023 Jan 12;49(2):43. doi: 10.3892/or.2023.8480

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Copyright: © Lim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of ACT-3 on the cell cycle distribution in MCF-7 cell lines. (A) Cells were treated with the indicated concentrations of ACT-3 and SAHA for 48 h, stained with propidium iodide (PI) and analyzed via flow cytometry to determine the cell cycle distribution. (B and C) Cells were treated with the vehicle control, ACT-3 (2, 4 and 8 µM), or SAHA (5 µM) for 48 h and the expression levels of cell cycle distribution-related proteins were determined via western blotting. β-actin was used as a loading control. The data are expressed as the mean ± SD of duplicate experiments. Statistical analysis was performed using one-way analysis of the variance (ANOVA), followed by Bonferroni's multiple comparison tests. *P<0.05 and **P<0.01 vs. the control. ACT-3, 3β,6β-dihydroxyurs-12-en-27-oic acid; SAHA, suberoylanilide hydroxamic acid.