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. 2023 Jan 12;49(2):43. doi: 10.3892/or.2023.8480

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Copyright: © Lim et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Effect of ACT-3 on MCF-7 cell migration. (A and B) MCF-7 cells were treated with the indicated concentrations of ACT-3 and SAHA. Wound images were obtained daily using IncuCyte ZOOM and scratch density was determined by using IncuCyte software. Statistical analysis was performed using one-way analysis of the variance (ANOVA), followed by Bonferroni's multiple comparison tests. *P<0.05 and **P<0.01 vs. the control. (C and D) Cells were treated with the indicated concentrations of ACT-3 and SAHA. The expression levels of cell migration-related proteins were determined via western blotting. β-actin was used as a loading control. ACT-3, 3β,6β-dihydroxyurs-12-en-27-oic acid; SAHA, suberoylanilide hydroxamic acid; TIMP, tissue inhibitor of metalloproteinase.