Table 2.
High-confidence non-introgressable j-blocks for the identification of genes involved in speciation.
| > = 1 j-sites | > = 2 j-sites | ||||
|---|---|---|---|---|---|
| No. of j-blocks (No. scaffolds with j-blocks) | 1189 (184) | 168 (44) | |||
| Length of j-blocks—Range (mean) |
3 bp–43.51 Kb (1 010 bp) |
23 bp–35.75 Kb (1 062 bp) |
|||
| No. of j-sites in a block—Range (total j-sites) | 1–6 (1443) | 2–6 (422) | |||
| Total length of j-blocks (% of the genome) | 1 201 823 bp (0.51%) | 178 520 bp (0.075%) | |||
| No. of genes with j-sites | 171 | 19 | |||
| No. of genes of flower development with j-sites | – | 6 (see below) | |||
| Gene name | j-sites | L(aa) | Site a | Function in Arabidopsis thaliana | |
|
RM_76773.10
(AT2G14110) |
3 | 255 | 4 | Haloacid dehalogenase-like hydrolase (HAD) superfamily protein. Participating in pollen germination and tube growth [54]. | |
|
RM_76929.10
(AT1G55490) |
2 | 294 | 3 | CPN60B. Mutants in this gene develops lesions on its leaves and accelerated cell death due to heat shock stress. Participating in embryo and seed development [57]. | |
|
RM_76979.9
(AT3G15510) |
2 | 199 | 1 | NAC2 (NAC domain containing protein 2). Involved in the regulation of stamen development and embryonic development [55,58]. | |
|
RM_77078.7
(AT5G11530) |
3 | 1415 | 1 | EMF1 (embryonic flower 1). Involved in regulating reproductive development [52,53]. | |
|
RM_77333.68
(AT1G08520) |
2 | 755 | 1 | ALB1. Encoding the CHLD subunit of the Mg-chelatase enzyme which is involved in chlorophyll biosynthesis. Participating in embryo and seed development [59]. | |
|
RM_77530.24
(AT3G05420) |
2 | 671 | 2 | ACBP4 (acyl-CoA binding protein 4). Expressed and function in floral lipid metabolism. Playing combinatory roles in pollen development [56]. | |
A j-block, unless explicitly stated, has > = 2 non-introgressable sites (j-sites). j-sites: the number of non-introgressable sites within the gene. L(aa): amino acid sequence length of the gene. aSite: No. of highly differentiated amino acids between R. mucronata and R. stylosa are given (see Supplementary Fig. S16).