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. 2001 Sep;69(9):5742–5751. doi: 10.1128/IAI.69.9.5742-5751.2001

FIG. 5.

FIG. 5

Escape of wild-type and dksA mutant bacteria from the Henle cell vacuole. Henle cells in which the cell membrane had been labeled with 5- and 6-carboxytetramethylrhodamine succinimidyl ester were infected with E. coli DH5α/pLR56 (InvA+), wild-type S. flexneri SA100, or dksA mutant SA5287. Each strain contained plasmid pUC-GFP to provide constitutive production of GFP. (A) GFP-labeled intracellular bacteria. (B) Rhodamine labeling of the Henle cell and vacuole membrane. (C) Overlay of the two images created in Adobe Photoshop.