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. Author manuscript; available in PMC: 2023 Mar 30.
Published in final edited form as: Sci Transl Med. 2022 Mar 30;14(638):eabl6328. doi: 10.1126/scitranslmed.abl6328

Fig. 3. PI3Kγ MP elevates airway cAMP abundance in vivo in mice.

Fig. 3.

(A) cAMP concentrations in tissues from BALB/c mice 24 hours after intratracheal instillation of different doses of PI3Kγ MP (0-750 μg/kg). Values in brackets indicate the dose of PI3Kγ MP expressed as μg/kg. The number of mice (n) ranged from 3 to 9 per group. (B) Tissue distribution of a FITC-labeled version of PI3Kγ MP at indicated time points after intratracheal instillation of 0.08 mg/kg (1.5 μg) in BALB/c mice. Representative images of n=3 experiments are shown. Scale bar: 50 μm. (C) Amount of cAMP in tissues from mice treated as in (B). The number of mice (n) ranged from 3 to 6 per group. In (A), *P<0.05, **P<0.01, ***P<0.001 by one-way ANOVA followed by Bonferroni’s post-hoc test. In (C), *P<0.05 by Kruskal Wallis test followed by Dunn’s multiple comparison test. Throughout, data are mean ± s.e.m.