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. Author manuscript; available in PMC: 2023 Mar 30.
Published in final edited form as: Sci Transl Med. 2022 Mar 30;14(638):eabl6328. doi: 10.1126/scitranslmed.abl6328

Fig. 8. PI3Kγ MP enhances the effect of elexacaftor/tezacaftor/ivacaftor in primary F508del/F508del HBE cells.

Fig. 8.

(A) Representative traces of ISC in primary human CF bronchial epithelial cells from a donor with CF (Patient BE93) homozygous for the F508del mutation (F508del/F508del HBE) and grown at the air-liquid interface (ALI). Cells were corrected for 24 hours with VX-661 and VX-445 alone (10μM+3μM) or together with PI3Kγ MP (10 μM), before exposure at the indicated time to the following drugs: Amiloride (100 μM), VX-770 (1 μM), CPT-cAMP (100 μM), and CFTRinh-172 (10 μM). (B) Average total current variation in response to VX-770 (1 μM) from n=5-8 technical replicates of donor BE93. (C) Average total current variation in response to VX-770 (1 μM) from n=5 technical replicates of a second F508del/F508del donor (patient BE91). Representative ISC traces are provided in Fig. S9E. Throughout, *P<0.05 and **P<0.001 and by Student’s t test. Data are mean ± SEM.