(A) Representative SPR traces of the adsorption of supercharged
albumins and native BSA to perfluorodecanethiol monolayers modeling
fluorinated oil interfaces. (B) Corresponding quantification of resulting
protein surface densities. (C) SPR quantification of FN or collagen
type I (Col I) adsorption at the surface of supercharged protein layers.
(D) Corresponding protein surface densities. (E) Epifluorescence microscopy
images of FN or Col I adsorption onto BSA, cBSA, and aBSA emulsions;
green, FN; red, Col I. Scale bars, 200 μm. (F) Quantification
(mean fluorescence intensity) of adsorbed FN or Col I on corresponding
protein nanosheets. Error bars are s.e.m.; n = 3.