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. 2023 Jan 9;10:1094124. doi: 10.3389/fcell.2022.1094124

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Copyright © 2023 Marr, Zamboulis, Werling, Felder, Dudhia, Pitsillides and Thorpe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Canonical and network-predicted vascular and basement membrane components were enriched within the interfascicular matrix. Immunohistochemical labelling of longitudinal sections confirmed interfascicular expression of Type IV collagen (A), full-length laminin (B), Perlecan (C), as well as vascular markers EMCN (D) and VWF (E). Immunolabelling validation also confirmed enrichment within interfascicular vasculature with network-predicted markers DAG1 (IIH6; (F), CD133 (G), ITGB1 (H), laminin isoform LAMA5 (I), and angiogenic mediators NTN1 (J) and NRP1 (K). Scale bar = 75 µm. Representative images shown.