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. 2023 Jan 9;10:1094124. doi: 10.3389/fcell.2022.1094124

FIGURE 5.

FIGURE 5

CD146+ cells exhibit limited clonogenicity and lineage potential. Representative images of colonies formed by MSCs, TDCs, CD146- and CD146+ populations (A–D). Scale bar = 1 cm. Oil Red O staining of MSCs, TDCs, CD146- and CD146+ cells (E–L) under adipogenic conditions using StemPro® Adipogenesis differentiation media (E–H) and control conditions (I–L) demonstrate that TDCs, CD146+ and CD146− cells produce a limited number of lipid vesicles. Lipid vesicles = red. n = 3 per cell type (biological replicates). n = 3 per condition (technical replicates). Scale bar = 100 µm. Alizarin Red S staining of MSCs, TDCs, CD146- and CD146+ cells (M–T) under osteogenic conditions containing 2 mM DiP (M–P) and control conditions (Q–T) demonstrate that tendon cells exhibit limited mineralisation capacity when separated. Mineralised nodules = black. Calcium deposits = red. Unmineralised matrix = reflective/white. Scale bar = 100 µm. Images shown are representative of each condition.