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. 2023 Jan 11;51(2):16. doi: 10.3892/ijmm.2023.5219

Figure 2.

Figure 2

miR-212-3p negatively regulates FGF21/CHD1 to promote lipogenesis. (A and B) FGF21 was knocked down using FGF21-siRNA. (C and D) CHD1 was knocked down using CHD1-siRNA. (E and F) The decrease in FASN expression caused by transfection with miR-212-3p inhibitor alone was blocked by FGF21-siRNA. (G and H) The decrease in FASN expression caused by transfection with miR-212-3p inhibitor alone was blocked by CHD1-siRNA. (I-L) The knockdown of FGF21/CHD1 abolished the inhibitory effects of miR-212-3p inhibitor on lipogenesis, as evidenced by Nile Red staining and TG content detection. The data are presented as the mean ± SEM. *P<0.05, **P<0.01. FGF21, fibroblast growth factor 21; CHD1, chromodomain helicase DNA binding protein 1; FASN, fatty acid synthase; TG, triglyceride.