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. 2023 Jan 11;51(2):16. doi: 10.3892/ijmm.2023.5219

Figure 6.

Figure 6

Exercise improves lipid accumulation by inhibiting miR-212-3p via activating EGR1. (A) Schematic diagram of miR-212-3p promoter predictions. (B) Mammalian phenotype analysis. (C-F) miR-212-3p expression decreased and FGF21 expression increased upon EGR1 overexpression. (G-J) miR-212-3p expression increased and FGF21 expression decreased upon EGR1 overexpression. (K, L) The corresponding elevation of FGF21 by the overexpression of EGR1 was blocked at the protein level by co-transfection of miR-212-3p mimics. (M-O) The overexpression of miR-212-3p abolished the inhibitory effect of EGR1-OE in lipogenesis as evidenced using Nile Red staining and TG content detection. Data are from three independent experiments. The data are presented as the mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001. EGR1, early growth response 1; FGF21, fibroblast growth factor 21; OE, overexpression; SI, siRNA; FFA, free fatty acid.