Skip to main content
. Author manuscript; available in PMC: 2023 Dec 6.
Published in final edited form as: Sci Signal. 2022 Dec 6;15(763):eabn2743. doi: 10.1126/scisignal.abn2743

Figure 4. TSC2 reconstitution reduces YAP/TAZ accumulation and fibronectin production, suppresses proliferation, and induces apoptosis in human PAH PAVSMCs.

Figure 4.

a, b. Human PAH PAVSMCs were transfected with GFP or GFP-TSC2 for the indicated times and immunoblotted to detect the indicated proteins. Data are means±SE from 3 subjects/group. P values for GFP-TSC2 compared to GFP were determined by Kruskal-Wallis rank test with Dunn’s pairwise comparison.

c-e. Proliferation (as measured BrdU incorporation) (c), apoptosis (as measured by TUNEL staining) (d), and immunocytochemical analysis to detect cleaved caspase 3 (red), GFP (green), and DAPI (blue) (e). Data are means±SE from 3 subjects/group. A minimum of 12 transfected cells/subject and condition were analyzed. P values for GFP-TSC2 compared to GFP were determined by Mann Whitney U test. Scale bar, 50 μm. White arrows indicate transfected cells.