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. 2022 Dec 1;43(2):267–285. doi: 10.1161/ATVBAHA.122.317800

Figure 2.

Figure 2.

Neutrophils protect against infective endocarditis. Following neutrophil depletion, mice underwent endocarditis surgery and were monitored for 1 day. A, Schematic overview of the neutrophil depletion experiment design (made with Biorender.com). B–D, Proportions of mice that developed infective endocarditis (red) at day 1 in neutrophil-depleted (anti-Ly6G) mice compared with isotype-control mice, infected with the methicillin-resistant strain of Staphylococcus aureus USA300, a clinical endocarditis isolate S. aureus strain and a coagulase-negative strain (Staphylococcus epidermidis). E–G, Bacteremia levels at end point with corresponding median (interquartile range) in neutrophil-depleted (n=20, 14, 10) and isotype-control (n=20, 18, 10) mice. Endocarditis vegetations are depicted in red, sterile thrombi in orange, and no thrombus in black. H–J, Proportions of mice that developed sterile thrombi (orange) in neutrophil-depleted mice compared with isotype-control mice. Fisher Exact (B–D, H–J) or Mann-Whitney tests (E–G) were conducted. CFU indicates colony-forming units; and Ly6G, lymphocyte antigen 6 complex locus G6D.