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. 2022 Dec 1;43(2):267–285. doi: 10.1161/ATVBAHA.122.317800

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© 2022 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Neutrophils undergoing NET (neutrophil extracellular trap) formation and released NETs are present in mice with Staphylococcus aureus–induced endocarditis. A–C, Fluorescence microscopy images of the following NET markers: H3Cit (citrullinated histone H3, green), MPO (myeloperoxidase, red), and DNA (blue) in inflammation-induced endocarditis vegetations infected with S. aureus USA300 (n=8, A), Newman (n=6, B) or a clinical S. aureus endocarditis strain (n=25, C) at day 3. Scale bar of upper and lower panels equals 200 μm and 50 μm respectively. In C, a different color balance threshold was used for MPO than in A and B. D and E, Quantifications of MPO (D) and H3Cit (E) positive area in mice with infective endocarditis (red dots, n=8, 6, 25) compared with mice without a vegetation (black dots, n=7, 7, 18). Median (interquartile range) are represented, and Mann-Whitney tests were used to test for statistical significance (D and E).