Skip to main content
. 2022 Dec 22;7(24):e155147. doi: 10.1172/jci.insight.155147

Figure 7. Complex I–supported mitochondrial respiration in quadriceps muscle of C26 tumor–bearing mice.

Figure 7

ADP-stimulated (state III) respiration, supported by complex I–supported (NADH) substrates pyruvate (5 mM) and malate (2 mM), was assessed in the absence (-creatine) and presence (+creatine) of 20 mM creatine at a range of [ADP] until maximal respiration was achieved to model a spectrum of metabolic demands. Respiration was assessed in the quadriceps normalized to bundle size at 2 weeks (A and B) and normalized to ETC subunit content (C and D) to permit comparisons of intrinsic mitochondrial respiratory responses in each group. Creatine sensitivity was assessed by calculating the +creatine/-creatine ratio (E and F) given creatine normally increases ADP-stimulated respiration. The same measurements were completed at 4 weeks (G–L). Results represent mean ± SD; n = 8–16; a 2-way ANOVA was used to determine the difference between PBS(2wk) versus C26(2wk) and PBS (4wk) versus C26(4wk). #P < 0.05, PBS(2wk) versus C26(2wk); *P < 0.05, PBS(4wk) versus C26(4wk).