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. 2023 Jan 20;38(1):2155814. doi: 10.1080/14756366.2022.2155814

Table 4.

Cytotoxic activity of the target compounds against MCF-7, HCT-116, HepG-2, MCF-7/ADR cancer cell lines and normal cell lines, HFL-1, WI-38.

Inline graphic
IC50 µMa
    Cancerotoxicity
Normotoxicity
Cpd. R MCF-7 HCT-116 HepG-2 MCF-7/ADR HFL-1 WI-38
4a 4-F 16.5 ± 0.1 6.4 ± 0.1 11.3 ± 0.1 24.4 ± 1.2 45.1 ± 0.2 56.7 ± 1.1
4b 2-Cl 11.3 ± 0.1 2.5 ± 0.2 10.7 ± 0.5 13.3 ± 0.5 71.9 ± 0.1 66.7 ± 0.2
4c 3-Cl 8.5 ± 0.6 9.9 ± 0.1 11.0 ± 0.2 10.9 ± 0.2 59.5 ± 1.1 61.5 ± 1.2
4d 4-Cl 0.6 ± 0.2 1.9 ± 0.4 7.2 ± 0.1 12.5 ± 0.3 77.1 ± 1.1 83.0 ± 1.1
4e 2-Br 8.3 ± 0.6 5.9 ± 0.4 4.9 ± 0.2 49.5 ± 1.1 59.1 ± 1.1
4f 3-Br 69.2 ± 0.3 11.5 ± 1.1 63.6 ± 3.2
4g 4-Br 5.6 ± 0.1 2.2 ± 0.1 2.0 ± 0.1 19.6 ± 1.0 65.3 ± 0.1 69.0 ± 1.1
4h 4-I 48.4 ± 0.1 27.7 ± 0.3 39.2 ± 0.2
4i 4-Me 7.0 ± 0.1 2.9 ± 0.1 8.8 ± 0.2 42.8 ± 2.4 71.6 ± 0.3 61.7 ± 0.1
4j 4-CF3 26.8 ± 1.8 20.2 ± 1.1 5.3 ± 0.1
4k 2,4-F2 3.0 ± 0.3 4.1 ± 0.2 8.2 ± 0.6 11.5 ± 0.6 59.6 ± 0.1 47.3 ± 0.2
4l 2,6-F2 15.4 ± 0.2 77.7 ± 0.3 28.5 ± 0.4
4m 2,3-Cl2 9.1 ± 0.2 42.0 ± 0.2 11.8 ± 0.2
4n 2,4-Cl2 1.8 ± 0.1 1.7 ± 0.2 7.3 ± 0.2 15.5 ± 0.2 71.7 ± 0.1 61.9 ± 0.3
4o 2,5-Cl2 3.8 ± 0.3 8.3 ± 0.2 46.6 ± 0.1
4p 2,6-Cl2 41.5 ± 0.1 18.9 ± 0.2 25.9 ± 0.3
4q 3,4-Cl2 2.3 ± 0.1 1.3 ± 0.1 1.5 ± 0.1 13.4 ± 0.4 75.8 ± 0.2 72.8 ± 0.4
4r 2,5-Br2 4.9 ± 0.5 5.3 ± 0.4 6.8 ± 0.5 38.1 ± 0.2 33.9 ± 0.4
4s 3,5-Br2 13.6 ± 0.2 15.0 ± 2.6 13.4 ± 1.1
4t 2-Cl-6-F 100.0 ± 1.3 96.5 ± 2.0 48.1 ± 1.3
4u 2-F-4-CF3 0.2 ± 1.3 14.7 ± 2.3 10.6 ± 0.9 19.7 ± 0.8 55.3 ± 0.1 60.3 ± 0.1
4v 2,4-(OMe)2 100.0 ± 0.2 49.9 ± 0.5 93.2 ± 0.2
4w 3,4-(OMe)2 2.6 ± 0.5 6.2 ± 0.3 52.3 ± 0.4 11.5 ± 0.6 47.1 ± 0.2 52.9 ± 0.3
4x 2,3,4-(OMe)3 96.1 ± 0.6 36.6 ± 0.1 59.7 ± 0.3
4y 3,4,5-(OMe)3 26.1 ± 0.1 15.4 ± 0.1 8.8 ± 0.2 34.1 ± 0.2 29.9 ± 0.4
4z 3,5-Br2-2-OMe 5.8 ± 0.04 6.8 ± 0.1 3.5 ± 0.2 33.3 ± 0.1 39.6 ± 0.4
A 7.5 ± 0.03 3.2 ± 0.1 5.7 ± 0.1
B 0.8 ± 0.01 0.9 ± 0.02 1.7 ± 0.4 18.6 ± 0.2

aIC50 values expressed in µM as the mean values of triplicate wells from at least three experiments and are reported as the mean ± standard error, A = Vinblastine and B = Doxorubicin.