Figure 5. SMC-specific deficiency of CCN2 represses SMC contractile markers in vivo.

(A) qPCR analysis of various mRNA expression in abdominal aortic lysates from CCN2^fl/fl and CCN2^SMCΔ mice with 7-day Ang II infusion (n = 5). *P < 0.05, ***P < 0.001, ****P < 0.0001, 2-tailed Student’s t test. (B) Western blot analysis of various protein expression in abdominal aortic lysates from mice in each group (n = 4). (C) Quantification of protein expression in B. Target protein levels were normalized to Vinculin. Data were quantified and represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (effect of CCN2 deficiency within same treatment group); ^#P < 0.05, ^##P < 0.01 (effect of Ang II within genotype group); 2-way ANOVA.