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Western blot analysis of various protein expression in cultured human primary aortic SMCs (HASMCs) with CCN2 deficiency (Ad-CCN2-shRNA versus Ad-scrmb-shRNA) (A) or overexpression (Ad-GFP-CCN2 versus Ad-GFP) (B). Target protein levels were normalized to GAPDH. Data were quantified and represented as mean ± SEM. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, 2-tailed Student’s t test.
