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. 2023 Jan 10;8(1):e158937. doi: 10.1172/jci.insight.158937

Figure 5. Pairwise single-cell transcriptomic comparisons of preculture 1° AEC2s versus cultured AEC2s.

Figure 5

(A) Heatmap of top 25 upregulated and top 25 downregulated genes comparing preculture 1° AEC2s to cultured 1° AEC2s by scRNA-Seq (ranked by average log fold change, FDR < 0.05; row-normalized expression z scores). (B) GSEA (camera using Hallmark gene sets) of differentially regulated gene sets in preculture 1° AEC2s versus cultured AEC2s (cultured 1° AEC2s and feeder-free iAEC2s combined; FDR < 0.05).