Skip to main content
. 2023 Jan 10;8(1):e160578. doi: 10.1172/jci.insight.160578

Figure 5. Minocycline has lasting detrimental effects on the skeleton and liver.

Figure 5

Female C57BL/6T specific pathogen–free (SPF) mice were administered vehicle control (VEH) or minocycline (MINO) from age 6 to 12 weeks; euthanized at (A–K) age 12 weeks and (L–V) age 18 weeks. qRT-PCR 16s rDNA analysis of colonic contents evaluating bacterial load in (A) 12-week-old mice and (L) 18-week-old mice; n = 6/group. Bacterial load determined by normalizing the universal 16S gene to a bacterial DNA standard; quantification by the 2-ΔCT method. qRT-PCR 16s rDNA analysis of colonic contents evaluating bacterial phyla in (B) 12-week-old mice and (M) 18-week-old mice; n = 6/group. Phylum outcomes determined by normalizing phyla genes to the universal 16S gene; quantification via the 2-ΔΔCT method. Micro-CT analysis of distal femur trabecular bone in (C–E) 12-week-old mice (n = 6/group) and (N–P) 18-week-old mice (n = 5/group): (C and N) representative images; (D and O) bone volume per tissue volume (BV/TV); (E and P) trabecular bone mineral density (Tb.BMD). Dynamic histomorphometric analysis of trabecular bone formation indexes in L4 vertebra of (F–H) 12-week-old mice and (Q–S) 18-week-old mice; calcein administered 5 and 2 days prior to sacrifice; n = 5–6/group: (F and Q) representative images (original magnification, 200×); (G and R) mineral apposition rate (MAR); (H and S) bone formation rate (BFR). N-terminal propeptide of type 1 procollagen (P1NP) serum ELISA in (I) 12-week-old mice (n = 5–6/group) and (T) 18-week-old mice (n = 4–5/group). Periodic acid–Schiff–stained (PAS-stained) median liver lobe sections in (J and K) 12-week-old mice and (U and V) 18-week-old mice; n = 4–5/group: (J and U) representative images (original magnification, 200×), (K and V) PAS+ area per tissue area (%). Unpaired 2-tailed t test in 12-week-old mice and 18-week-old mice; reported as mean ± SEM; *P < 0.05 vs. VEH, **P < 0.01 vs. VEH, ***P < 0.001 vs. VEH.