Fig 1. Heatmap with euclidean distance and ward clustering of mean serum metabolites determined by quantitative NMR spectroscopy in overweight cats (n = 14) receiving control (no additional choline supplementation, 1.2 x NRC RA, 77 mg/kg BW0.67), choline at 2 x NRC RA (126 mg/kg BW0.67), 4 x NRC RA (252 mg/kg BW0.67), 6 x NRC RA (378 mg/kg BW0.67), and 8 x NRC RA (504 mg/kg BW0.67), in a 5 x 5 Latin square design for 3-week periods, with a significant effect of choline dose (P < 0.05).
