. 2023 Jan 9;9:1080965. doi: 10.3389/fcvm.2022.1080965

TABLE 2.

Mutations that have been reported to cause uncoupling.

Mutation	Effect of phosphorylation on Ca²⁺-sensitivity, pCa₅₀ uP- pCa₅₀ P	Measurement method	Publication
*DCM*
ACTC E361G	0.017	In vitro motility assay (IVMA)	(37, 38)
TPM1 E54K	0.021	IVMA	(38)
TPM1 E40K	0.00	IVMA	(38)
TPM1 D230N	-0.013	IVMA	(38)
TNNC1 G159D	-0.013	IVMA/Ca²⁺ binding	(29, 30, 38, 39)
TNNC1 Y5H	0.068	Skinned fiber	(40)
TNNC1 M103I	0.017	Skinned fiber	(40)
TNNC1 I148V	0.053	Skinned fiber	(40)
TNNT2 ΔK210	-0.009	IVMA/skinned fiber	(38, 41, 42)
TNNT2 R141W	-0.022	IVMA	(38)
TNNI3 K36Q	-0.009	IVMA/ATPase	(38, 43)
*HCM*
ACTC E99K	-0.004	IVMA	(44, 45)
TPM1 E180G	-0.009	Skinned fiber	(45, 46)
TNNC1 L29Q	-0.036	Ca²⁺ binding/ATPase	(30, 47, 48)
TNNT2 R92Q	0.041	IVMA	(49)
TNNT2 Δ14	0.000	IVMA	(49)
TNNT2 Δ28 + 7	0.000	IVMA	(49)
TNNT2 ΔE160Q	0.000	IVMA	(49)
TNNT2 S179F	0.041	IVMA	(49)
TNNT2 K273E	0.041	IVMA	(49)
TNNT2K280N	0.041	IVMA	(49, 50)
TNNI3 R145G	0.000	IVMA/ATPase	(27, 51)
TNNI3 R145W	0.045	IVMA/ATPase	(52)
TNNI3 P83S	0.000	Exchanged myofibrils	(53)
TNNI3 R21C	0.049	Skinned fiber	(35, 51)
TNNI3 G203S	0.057	ATPase/IVMA	(28)
TNNI3 K206Q	0.000	ATPase/IVMA	(28)