TABLE 1.
Bacterial strains, plasmids, and bacteriophages used in this study
| Strain, plasmid, or bacteriophage | Descriptiona | Reference or source |
|---|---|---|
| E. coli | ||
| 260-1 | LT CFA/III | 12 |
| 31-10 | LT CFA/III | 13 |
| 31-10P | CFA/III-negative spontaneous derivative of strain 31-10; LT | This study |
| HB101 | Host for recombinant plasmids | Laboratory stock |
| JM109 | Host for recombinant M13 bacteriophages | Laboratory stock |
| Plasmid | ||
| pSH1134 | 32.5-kb plasmid containing CFA/III gene cluster and Tn3 insertion; Apr | 32 |
| pTT201 | pMW119 (AccI and BamHI sites) containing 8.6-kb ClaI1-BamHI1 fragment from pTT202; Apr | 40 |
| pTT202 | pMW119 (AccI site) containing 11.5-kb ClaI1-ClaI2 fragment from pSH1134; Apr | 32, 40 |
| pTT206 | pACYC184 (BamHI site) containing 12.4-kb BamHI1-BamHI2 fragment from pSH1134; Cmr | 32, 41 |
| pTT222 | pACYC184 (EcoRI site) containing 5.2-kb EcoRI2-EcoRI3 fragment from pTT206; Tcr | 41 |
| pTT224 | pACYC184 (SalI and BamHI sites) containing 7.6-kb SalI4-BamHI2 fragment from pTT206; Tcr | 41 |
| pTT237 | pMW119 (KpnI and AccI sites) containing 9.0-kb KpnI-ClaI2 fragment from pTT202; Apr | This study |
| pMW119 | Cloning vector; AprlacZ | Nippon Gene |
| pACYC184 | Cloning vector; Cmr Tcr | Nippon Gene |
| Bacteriophage | ||
| M13mp18 | Cloning vector; lacZ | Laboratory stock |
| M13mp19 | Cloning vector; lacZ | Laboratory stock |
a
Apr, Cmr, and Tcr, ampicillin, chloramphenicol, and tetracycline resistance, respectively.