TABLE 2.
Features of the cof genes and deduced proteins
| Gene
|
Deduced protein
|
||||||
|---|---|---|---|---|---|---|---|
| Name | Position (start→stop) | Length (bp) | G+C content (%) | Length (amino acids) | Size (kDa) | Predicted function or location | Closest homology by FASTA (% identity, overlap [no. of amino acids]) |
| cofR | 635→935 | 300 | 33.2 | 100 | 11.7 | Regulation | Serovar Typhimurium PefB (53.8, 80); ETEC FaeB (48.1, 79) |
| cofS | 1228→2077 | 849 | 39.2 | 283 | 32.0 | Regulation | ETEC FapR (25.0, 260); ETEC CfaD (24.6, 264) |
| cofT | 2092→2533 | 441 | 43.8 | 147 | 16.7 | Unknown | Serovar Typhi IagB (41.2, 136); EPEC BfpH (31.2, 138) |
| cofA | 2836→3550 | 714 | 42.3 | 238 | 25.3 | Type IV pilin | ETEC LngA (78.2, 238); V. cholerae TcpA (34.7, 225) |
| cofB | 3613→5182 | 1,569 | 36.7 | 523 | 57.1 | Type IV pilin-like protein | V. cholerae TcpB (22.1, 77) |
| cofC | 5201→5612 | 411 | 34.2 | 137 | 15.7 | Unknown | EPEC BfpG (26.9, 134); V. cholerae TcpQ (24.3, 70) |
| cofD | 5632→7087 | 1,455 | 35.4 | 485 | 54.2 | Outer membrane lipoprotein | EPEC BfpB (24.3, 437); V. cholerae TcpC (22.5, 485) |
| cofE | 7091→7649 | 558 | 36.0 | 186 | 21.8 | Inner membrane | None |
| cofF | 7653→8478 | 825 | 32.8 | 275 | 31.2 | Inner membrane | V. cholerae TcpD (24.4, 270) |
| cofG | 8468→8951 | 483 | 39.2 | 161 | 17.7 | Unknown | None |
| cofH | 9151→10483 | 1,332 | 39.0 | 444 | 50.0 | Inner membrane nucleotide-binding protein | V. cholerae TcpT (44.2, 441); EPEC BfpD (26.9, 431) |
| cofI | 10469→11492 | 1,023 | 36.6 | 341 | 37.8 | Inner membrane | V. cholerae TcpE (38.0, 337); EPEC BfpE (23.6, 343) |
| cofJ | 11508→12552 | 1,044 | 35.4 | 348 | 39.6 | Unknown | None |
| cofP | 12557→13376 | 819 | 35.5 | 273 | 30.5 | Prepilin peptidase | V. cholerae TcpJ (35.7, 266); EPEC BfpP (31.7, 262) |