Skip to main content
. 2023 Jan 9;9:1072056. doi: 10.3389/fmed.2022.1072056

FIGURE 4.

FIGURE 4

Effects of PFEA and luteolin on the NLRP3 inflammasome pathway inhibition in Spike S1-exposed A549 lung cells. A549 lung cells were pre-treated with the PFEA at concentration of 0–100 μg/mL or luteolin at concentration of 0–20 μg/mL for 24 h, and then exposed to Spike S1 (100 ng/mL) for 3 h. The inhibitory effects of PFEA (A) and luteolin (B) on the expression of NLRP3, ASC, caspase-1 (p50), and the cleaved caspase-1 (p20) in the cell lysate of A549 cells were determined by western blot. The band density was measured using Image J software. Spike S1-exposed A549 cells are presented as 100% of control. Data are presented as mean ± S.D. values of three independent experiments, *p < 0.05, **p < 0.0,1 and ***p < 0.001 vs. the Spike S1-exposed A549 cells.